cytotoxic concentration cc50  (SYSTAT)

Journal: Journal of Virology

Article Title: An RNA replicon system to investigate promising inhibitors of feline coronavirus

doi: 10.1128/jvi.01216-23

Figure Lengend Snippet: Individual tested antiviral compounds. BHK-F-Rep cells treated with ( A ) Remdesivir, ( B ) GC376, ( C ) Nirmatrelvir, and ( D ) Amodiaquine for 96 hours and analyzed by flow cytometry for GFP expression as shown in . Relative inhibition was calculated by the median intensity of GFP expression in the cells treated with antiviral drugs or DMSO in comparison to mock control. In addition, IC50 values and CC50 values were calculated as explained in the methods and represented on the individual graphs. ( A–C ) Remdesivir, GC376, and Nirmatrelvir showed effective antiviral dose-response with IC50 values < 1 µM, at concentrations lower than the cytotoxic concentrations ( D ) Amodiaquine alone did not show any inhibitory effect at the concentrations that already affected the cell growth The black line in the graphs represents the mean value of the data collected from three independent experiments, and the gray area represents standard error of the mean.

Article Snippet: Importantly, the 50% cytotoxic concentration (CC50) values of these compounds were 99.1 µM for Remdesivir and beyond the highest concentration used (200 µM) for GC376 and Nirmatrelvir ( ; ).

Techniques: Flow Cytometry, Expressing, Inhibition, Comparison

Journal: Journal of Virology

Article Title: An RNA replicon system to investigate promising inhibitors of feline coronavirus

doi: 10.1128/jvi.01216-23

Figure Lengend Snippet: IC50, CC50, and SI values for antiviral compounds and combination of compounds on BHK-F-Rep cells

Article Snippet: Importantly, the 50% cytotoxic concentration (CC50) values of these compounds were 99.1 µM for Remdesivir and beyond the highest concentration used (200 µM) for GC376 and Nirmatrelvir ( ; ).

Techniques:

Journal: Journal of Virology

Article Title: An RNA replicon system to investigate promising inhibitors of feline coronavirus

doi: 10.1128/jvi.01216-23

Figure Lengend Snippet: Comparison to virus infection. Testing of three different compounds or combinations of compounds: GC376 ( A ), Remdesivir ( B ), and Remdesivir + GC376 in a 1:1 ratio ( C ) in recFCoV-GFP-infected Fcwf-4 cells with dose-response curve of recFCoV-GFP infected Fcwf-4 cells treated for 48 hours. Read-out as with the BHK-F-Rep cells done via flow cytometry and detection of GFP-positive cells (relative Inhibition) including IC50 values are shown in black with the left y-axis. As with the BHK-F-Rep cells, data shown on cell viability testing in the recFCoV-GFP infected Fcwf-4 cells are shown in black with the right y-axis including CC50 values. Additional data on viral titers of the recFCoV-GFP infected Fcwf-4 cells treated with the inhibitors and corresponding IC50 values are shown in red. The black and red lines in the graphs represent the mean value of the data collected from three independent experiments of individual drugs or their combination, respectively. The gray and red shaded areas represent the standard error of the mean.

Article Snippet: Importantly, the 50% cytotoxic concentration (CC50) values of these compounds were 99.1 µM for Remdesivir and beyond the highest concentration used (200 µM) for GC376 and Nirmatrelvir ( ; ).

Techniques: Comparison, Virus, Infection, Flow Cytometry, Inhibition